lncRNA LOC100132354 promotes angiogenesis through VEGFA/VEGFR2 signaling pathway in lung adenocarcinoma

lncRNA LOC100132354 promotes angiogenesis through VEGFA/VEGFR2 signaling pathway in lung adenocarcinoma

Blog Article

Yumin Wang,1,* Fan Zhang,1,* Junjun Wang,1 Lijuan Hu,1 Feng Jiang,1 Jian Chen,1 Jie Chen,2 Liangxing Wang3 1Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou China; 2Intensive Center Unit, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China *These authors contributed equally to this work Purpose: This study aimed to Bosch DWB98JQ50B Serie 6 Built In 90cm 5 Speeds A+ Chimney Cooker Hood study the biological function and the molecular mechanisms associated with the promotion of angiogenesis by lncRNA LOC100132354 in lung adenocarcinoma (LAD).Patients and methods: The mRNA expression levels of 100 pairs of LAD and normal tissue samples of LOC100132354, vascular endothelial growth factor A (VEGFA), VEGF receptor-2 (VEGFR2), basic fibroblast growth factor (bFGF), and thrombospondin-1 (TSP-1) were analyzed by qPCR.LOC100132354 was knockdown and overexpressed in SPCA-1 and A549 cell lines to analyze the protein and mRNA expression levels of VEGFA, VEGFR2, bFGF, TSP-1, and changes in protein expression levels of Ras, P-A-Raf, P-B-Raf, P-C-Raf, P-Mekl/2, and P-Erk1/2.Tumor microvessel density (MVD) was analyzed in experimental nude mice.Results: The qPCR results Door Latch Hook showed that the mRNA expression levels of LOC100132354, VEGFA, VEGFR2, and bFGF mRNA in LAD tissues were significantly increased, while TSP-1 mRNA was significantly decreased compared with the adjacent tissues.

Survival analysis showed that VEGFA, VEGFR2, and bFGF were poor predictors, while TSP-1 was a good predictor in LAD.Knockdown or overexpression of LOC100132354 affected the expression levels of bFGF, VEGFA/VEGFR2 signaling pathway, and downstream target molecules, such as Ras, P-A-Raf, P-B-Raf, P-C-Raf, P-Mekl/2, and P-Erk1/2, while decreased TSP-1.After knockdown or overexpression of VEGFA expression, no significant changes in the expression level of LOC100132354 were found.Tumorigenesis of nude mice confirmed that LOC100132354 can significantly increase the tumor MVD.Conclusion: These findings suggest VEGFA was a downstream target gene of LOC100132354, promoting angiogenesis through VEGFA/VEGFR2 signaling pathway and downstream target molecules in LAD.

So, LOC100132354 is considered as an antiangiogenic target in LAD.Keywords: lung cancer, lncRNA, angiogenesis, signaling pathway.